报告题目: Radical-Mediated Peptide Tyrosine Nitration: From Fundamental, Bioanalytical to Neurodegenerative Proteomics
报告人: Ivan K. Chu💃，Department of Chemistry, University of Hong Kong
主持人: 石铁流 教授
报告时间: 11月24日 13:30 （周四）
报告地点: 天美娱乐534报告厅
报告人简介：
PROFESSIONAL CAREER
Jun 2008-now Associate Professor of Chemistry, The University of Hong Kong
Sep 2002-2008 Assistant Professor of Chemistry, The University of Hong Kong
2003-2007 Affiliate Scientist, Genome Research Centre, The University of Hong Kong
2005-9 Summer Visiting Scientist, Pacific Northwest National Laboratory, DOE, USA
2004 Summer Visiting Scientist, The Hebrew University of Jerusalem, Israel
2001-2002 Team Leader, LC/MS Scientist, MDS SCIEX, Toronto, Canada
2000-2001 Postdoctoral Fellow, Centre for Research in Mass Spectrometry, York University, Canada
EDUCATION
1995 University of Victoria, Canada, B.Sc.
2000 City University of Hong Kong in collaboration with National Research Council of Canada and York University, Canada, Ph.D.
报告内容👫：
Protein tyrosine nitration (PTN)—a hallmark of post-translational modification of proteins under nitrative stress in vivo—modification occurs regioselectively and site-specifically at diverse local sequences with no observed consensus modification motif. The mechanistic details governing the site-specificity of the ortho-tyrosine nitration are largely unknown. Here in, the mechanism of radical-mediated PTN has been elucidated in detail at the molecular level using an integrated approach combining gas phase synthesis of prototypical tyrosine-containing peptide radical cations,ion–molecule reactions with nitrogen dioxide, isotopic labelling MS experiments, and DFT calculations. To better understand the site-specific formation of endogenous PTNs, the fundamental factors governing the site-specificity of the 3-NT have been investigated using computationally tractable prototypical dityrosyl-containing peptides that mimic the local topological characteristics of peptides found in PTNs in vitro from a M. fascicularis model of cerebral ischemia;5 these nitrated peptides have been validated and shortlisted as potential selectivity determinants for the model peptides in their subsequent gas phase PTN reactions.
